Regulatory
precB
Part:BBa_K862003:Design
Designed by: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order) Group: iGEM12_Heidelberg_LSL (2012-06-08)
precB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the sequence was ordered on a synthetic oligo basis and cloned in front of EcoRI/XbaI reporter parts.
Source
E. coli MG1655 genome: http://ecoliwiki.net/colipedia/index.php/recB:Gene bp -70 to -1 before recB coding sequence.