Regulatory
precB

Part:BBa_K862003:Design

Designed by: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)   Group: iGEM12_Heidelberg_LSL   (2012-06-08)


precB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the sequence was ordered on a synthetic oligo basis and cloned in front of EcoRI/XbaI reporter parts.


Source

E. coli MG1655 genome: http://ecoliwiki.net/colipedia/index.php/recB:Gene bp -70 to -1 before recB coding sequence.

References